Effect of Puberty and Gender on Metabolic Hormones Level and Lipid Profile in Patients with Growth Hormone Deficiency
Keywords:
Growth hormone deficiency, metabolic hormones, puberty, genderAbstract
The current study aims to evaluate the effect of puberty and gender on metabolic hormones level and lipid profile in a sample with growth hormone deficiency (GHD). Seventy-five Iraqi patients with GHD (45 boys and 30 girls) within the age range (3-15) years were involved in this study. The study was achieved in the National Diabetic Center for Treatment and Research /Al-Mustansiriya University in a period of October, 2018 to April, 2019.
Blood samples were obtained from the patients to determine the level of basal GH before stimulation with clonidine, GH2 and GH3 after 1 h. and 1.30 h. stimulation with clonidine, respectively; insulin-like growth factor-1 (IGF-1); level of metabolic hormones [thyroid hormones: triiodothyronine (T3), tetraiodothyronine (thyroxin) (T4), thyroid stimulating hormone (TSH), and cortisol]; and lipid profile [cholesterol, triglycerides (TGs), high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL)].
Effects of the puberty on the studied parameters revealed that the levels of GH2, IGF-1, TGs and VLDL were significantly (P<0.05) higher in the pubertal group than the pre-pubertal group, while levels of T3 and T4 were significantly (P<0.01) lower in the pubertal group compared to the per-pubertal group. Non-significant (P>0.05) differences were found in level of other parameters (GH, GH3, TSH, cortisol, cholesterol, HDL and LDL) between the two groups. Effect of gender on the studied parameters showed non-significant (P>0.05) differences between boys and girls.
It can be concluded from this study that puberty effects on some parameters such as GH2, IGF-1, T3, T4, TGs and VLDL in patients with GHD; while gender shows no significant effect on the all studied parameters.
References
Lu, M., Flanagan, J. U., Langley, R. J., Hay, M. P., and Perry, J. K. 2019. Targeting growth hormone function: strategies and therapeutic applications. Signal Transduct. Tar., 4(1): 1-11.
Wille, N., Erhart, M., Petersen, C., and Ravens-Sieberer, U. 2008. The impact of overweight and obesity on health-related quality of life in childhood–results from an intervention study. BMC Public Health, 8(421): 1-9.
Zeller, M. H., and Modi, A. C. 2009. Development and initial validation of an obesity specific quality of life measure for children: sizing me up. Obesity, 17(6): 1171-1177.
Asakura, Y., Toyota, Y., Muroya, K., and Adachi, M. 2010. Growth hormone response to GH- releasing peptide-2 in children., J. Pediatr. Endocrinol. Metab. 23(5): 473-480.
Aguirre, G. A., De Ita, J. R., De La Garza, R. G., and Castilla-Cortazar, I. 2016. Insulin-like growth factor-1 deficiency and metabolic syndrome. J. Transl. Med., 14(3): 1-23.
Wrigley, S., Arafa, D., and Tropea, D. 2017. Insulin-like growth factor 1: at the crossroads of brain development and aging. Front. Cell. Neurosci., 11: 1-14.
Cabello, G., and Wrutniak, C. 1989. Thyroid hormone and growth: relationships with growth hormone effects and regulation. Reprod. Nutr. Dev., 29(4), 387-402.
Rousset, B.; Dupuy, C.; Miot, F. and Dumont, J. 2015. Thyroid hormone synthesis and secretion. South Dartmouth. MA. USA.
Dayan, C., and Panicker, V. 2018. Management of hypothyroidism with combination thyroxine (T4) and triiodothyronine (T3) hormone replacement in clinical practice: a review of suggested guidance. Thyroid Res., 11: 1-11.
Guyton, A. C. and Hall, J. E. 2006. Text Book of Medical Physiology. 12th ed. W.B. Saunders Company. pp. 1152.
Juster, R. P., McEwen, B. S., and Lupien, S. J. 2010. Allostatic load biomarkers of chronic stress and impact on health and cognition. Neuroscience and Biobehavioral Reviews, 35(1): 2-16.
Stratakis, C. A. 2006. Cortisol and growth hormone: clinical implications of a complex, dynamic relationship. Pediatr. Endocrinol. Rev., (3): 333-338.
Feingold, K. R., and Grunfeld, C. 2012. Lipids: a key player in the battle between the host and microorganisms. J. Lipid Res., 53(12): 2487-2489.
Mittendorfer, B., Yoshino, M., Patterson, B. W., and Klein, S. 2016. VLDL triglyceride kinetics in lean, overweight, and obese men and women. J. Clin. Endocrinol. Metab., 101(11): 4151-4160.
Johansen, T., Richelsen, B., Hansen, H. S., Din, N., and Malmlöf, K. (2003). Growth hormone- mediated break- down of body fat: effects of GH on lipases in adipose tissue and skeletal muscle of old rats fed different diets. Horm. Metab. Res., 35: 243–250
Clemmons, D. R. 2011. Consensus statement on the standardization and evaluation of growth hormone and insulin-like growth factor assays. Clin. Chem., 57(4): 555-559.
Racadot, A. 1991. Biosynthesis of thyroid hormones. Biochemical aspects. Immuno-Anal. Biol. Spe., 6(6): 27-32.
Burtis, C.A., Ashwood, E.R., and Bruns, D.E. 2012. Tietz Textbook of Clinical Chemistry and Molecular Diagnosis 5th ed. Elsevier, St. Louis, USA.
Nauck, M., Warnick, G.R. and Rifai, N. 2002. Methods for measurement of LDL-cholesterol: a critical assessment of direct measurement0T 0Tby homogeneous assays versus calculation. Clin. Chem., 48: 236-254.
SAS. 2012. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.
Bahrani, M.A., Ali, A.A., and Al-Samarraie, A.K.Y. 2011. Insulin-Like Growth Factor-1 (IGF-1) predicate the diagnosis of growth hormone deficiency in short prepubertal children, Iraqi J. Pharm. Sci., 20(2): 54-58.
Borges, M.D.F., Teixeira, F.C.C., Feltrin, A.K., Ribeiro, K.A., Nascentes, G.A.N., Resende, E.A.M. R., and Palhares, H.M.C. 2016. Clonidine-stimulated growth hormone concentrations (cut-off values) measured by immunochemiluminescent assay (ICMA) in children and adolescents with short stature. Clin. 71(4): 226-231.
Grote, F.K., Oostdijk, W., Keizer-Schrama, S.M.D.M., Van Dommelen, P., Van Buuren, S., Dekker, F.W., and Wit, J.M. 2008. The diagnostic works up of growth failure in secondary health care; an evaluation of consensus guidelines. BMC pediatrics, 8(1): 21.
Rogol, A.D., Roemmich, J.N., and Clark, P.A. 2002. Growth at puberty. J. Adolesc. Health, 31(6): 192-200.
Abdul Rahem, Z.A., Al-Sakkal, N.M., Al-Samarraie, A.Y. 2012. Role of insulin like growth factor (IGF-1) and its binding protein (IGFBP-3) in GH deficient short stature children. M.Sc. Thesis. College of Medicine. University of Mustansiriya.
Kanbur-ũksļz, N., Derman, O., and KŠnŠk, E. 2004. Correlation of sex steroids with IGF-1 and IGFBP-3 during different pubertal stages. Turk. J. Pediatr., 46: 315-321.
Taylor, P.N., Richmond, R., Davies, N., Sayers, A., Stevenson, K., Woltersdorf, W., and Okosieme,O. 2016. Paradoxical relationship between body mass index and thyroid hormone levels: a study using Mendelian randomization. J. Clini. Endocrinol. Metab. 101(2): 730-738.
Strich, D., Edri, S., and Gillis, D. 2012. Current normal values for TSH and FT3 in children are too low: evidence from over 11,000 samples. J. Pediatr. Endocrinol. Metab. 25(3): 245-248.
Witkowska-Sędek, E., Borowiecki, A., Majcher, A., Sobol, M., Rumińska, M., and Pyrżak, B. 2018. Thyroid function in children with growth hormone deficiency during long-term growth hormone replacement therapy. Cent. Eur. J. Immunol., 43(3): 255
Bülow P., I., Laurberg, P., Knudsen, N., Jørgensen, T., Perrild, H., Ovesen, L., and Rasmussen, L.B. 2005. A population study of the association between thyroid autoantibodies in serum and abnormalities in thyroid function and structure. Clin. Endocrinol., 62(6): 713-720.
King, L. S., Colich, N. L., LeMoult, J., Humphreys, K. L., Ordaz, S. J., Price, A. N., and Gotlib, I.
H. 2017. The impact of the severity of early life stress on diurnal cortisol: The role of puberty. Psychoneuroendocrinology, 77: 68-74.
Dismukes, A.R., Johnson, M.M., Vitacco, M.J., Iturri, F., and Shirtcliff, E.A. 2015. Coupling of the HPA and HPG axes in the context of early life adversity in incarcerated male adolescents. Dev. Psychobiol., 57(6):705-718.
Sas, T., Mulder, P., and Hokken-Koelega, A. 2000. Body composition, blood pressure, and lipid metabolism before and during long-term growth hormone (GH) treatment in children with short stature born small for gestational age either with or without GH deficiency. J. Clin. Endocrinol. Metab. 85(10): 3786-3792.
Gleeson, H.K., Souza, A.H., Gill, M.S., Wieringa, G.E., De A. Barretto, E.S., Barretto‐Filho, J.A.S., and Clayton, P. E. 2002. Lipid profiles in untreated severe congenital isolated growth hormone deficiency through the lifespan. Clin. Endocrinol. 57(1): 89-95.
Colao, A., Di Somma, C., Cuocolo, A., Spinelli, L., Tedesco, N., Pivonello, R., and Lombardi, G. 2001. Improved cardiovascular risk factors and cardiac performance after 12 months of growth hormone (GH) replacement in young adult patients with GH deficiency. J. Clin. Endocrinol. Metab., 86(5): 1874- 1881.
Eissa, M.A., Mihalopoulos, N.L., Holubkov, R., Dai, S., and Labarthe, D.R. 2016. Changes in fasting lipids during puberty. J. Pediatr., 170: 199-205.
Bittner, V. 2002. Lipoprotein abnormalities related to women’s health. Am. J. cardiol, 90(8), 77-84.
Cífková, R., and Krajčoviechová, A. 2015. Dyslipidemia and cardiovascular disease in women. Curr. Cardiol. Rep, 17(7): 52.
Bengtsson, B.A., Abs, R., Bennmarker, H., Monson, J.P., Feldt-Rasmussen, U., Hernberg-Ståhl, E., and Kims Study Group the Kims International Board. 1999. The effects of treatment and the individual responsiveness to growth hormone (GH) replacement therapy in 665 GH-deficient adults. J. Clin. Endocrinol. Metab., 84(11): 3929-3935.
Tsai, Y.Y., Rainey, W.E., and Bollag, W.B. 2017. Very low-density lipoprotein (VLDL)-induced signals mediating aldosterone production. J. endocrinol., 232: R115-R129.
Hughes, I.P., Choong, C.S., Cotterill, A., Harris, M., and Davies, P.S. 2010. Gender bias in children receiving growth hormone treatment. J. Clin. Endocrinol. Metab., 95(3): 1191-1198.
Hassan, H. A., Enright, W. J., Tucker, H. A., and Merkel, R. A. 2001. Estrogen and androgen elicit growth hormone release via dissimilar patterns of hypothalamic neuropeptide secretion. Steroids, 66(2): 71-80.
Mohammed, A. A., Barale, S. S., Dhotare, P. S., & Sonawane, K. D. (2025). Polymyxin-B as a Novel Inhibitor of Amyloid Beta Aggregation: Computational Insights and Experimental Validation. Journal of Molecular Structure, 143738.
Cook, D.M. 2004. Growth hormone and estrogen: a clinician's approach. J. Pediatr, Endocrinol. Metab., 17: 1273-1276.
Juul, A., Bang, P., Hertel, N.T., Main, K., Dalgaard, P., Jørgensen, K., and Skakkebæk, N.E. 1994. Serum insulin-like growth factor-I in 1030 healthy children, adolescents, and adults: relation to age, sex, stage of puberty, testicular size, and body mass index. J. Clin. Endocr. Metab., 78(3): 744-752.
Hussein, A. F., Khamees, H. H., Mohammed, A. A., Hussein, S. A. M., Ahmed, M. A., Saad, A., & Raoof, M. In-Silico Study Of Destabilizing Alzheimer’s Aβ42 Protofibrils With Curcumin.
Kapelari, K., Kirchlechner, C., Högler, W., Schweitzer, K., Virgolini, I., and Moncayo, R. 2008. Pediatric reference intervals for thyroid hormone levels from birth to adulthood: a retrospective study. BMC Endocr. Disord., 8(1): 15.
Mohammed, A. A., & Sonawane, K. D. (2022). Destabilizing Alzheimer's Aβ42 protofibrils with oleocanthal: In-silico approach. BIOINFOLET-A Quarterly Journal of Life Sciences, 19(3), 288-295.
Clark, P.A., and Rogol, A.D. (1996). Growth hormones and sex steroid interactions at puberty. Endocrinol. Metab. Clin. North Am., 25(3): 665-681.
Losa, M., Scavini, M., Gatti, E., Rossini, A., Madaschi, S., Formenti, I., and Lanzi, R. 2008. Long- term effects of growth hormone replacement therapy on thyroid function in adults with growth hormone deficiency. Thyroid, 18(12): 1249-1254.
Menon, P.S.N., Gupta, P., and Karmarkar, M.G. 1994. High and low dose clonidine tests for the diagnosis of growth hormone deficiency. Indian pediatrics, 31: 145-151.
Dorn, L.D., Burgess, E.S., Susman, E.J., Debellis, M.D., Gold, P.W., and Chrousos, G. P. 1996. Response to CRH in depressed and nondepressed adolescents: does gender make a difference? J. Adolesc. Child Acad. Am. J., 35(6): 764-773.
Lartey, A., Marquis, G.S., Aryeetey, R., and Nti, H. 2018. Lipid profile and dyslipidemia among school-age children in urban Ghana. BMC public health, 18(1): 320.
Ghannem, H., Darioli, R., Limam, K., Harrabi, I., Gaha, R., Trabelsi, L., and Bouslama, A. 2001. Epidemiology of cardiovascular risk factors among schoolchildren in Sousse, Tunisia. J. Cardiovasc. Risk., 8(2): 87-91.
