Evaluation Of IL-6 And IL-8 In the Saliva of Patients with Type II Diabetes Mellitus After Tooth Extraction
Keywords:
Interleukin-6, Interleukin-8, Tooth Extraction, Saliva, BOP, PI, GIAbstract
Type II diabetes mellitus (T2DM) is characterized by chronic low-grade inflammation and impaired wound healing, which may have undesirable effects on the outcomes of oral surgery. The expression of proinflammatory cytokines, including IL-6 and IL-8, is fundamental in immune regulation and for tissue damage or recovery. The purpose of the present investigation was to measure salivary IL-6 and IL-8 in patients with type II diabetes mellitus before and after undergoing tooth extraction, as well as in a control group of systemically healthy individuals. This was an observational case–control study that was performed at the college of dentistry university of Kufa, Al-Najaf, Iraq from April 2025 to December 2025. Sixty individuals participated in the study, including 30 T2DM patients and 30 gender- and age-matched healthy controls. Unstimulated whole saliva was collected one hour before extraction and two days after extraction. Enzyme linked immunosorbent assay (ELISA) was used to determine the levels of IL-6 and IL-8 in saliva. The statistical analysis between the two groups was analyzed by independent-samples and paired t-test with P < 0.05 considered as significant. At baseline, the concentrations of salivary IL-6 and IL-8 was significantly higher in patients with T2DM compared to controls (P < 0.05). Both cytokines were significantly elevated (P < 0.001) when compared with the basal values in diabetics on day 2 following tooth extraction; this difference was still statistically significant from that observed in controls (P < 0.05). Type II diabetes mellitus patients have a high salivary level of IL-6 and IL-8 at the baseline and an exaggerated response to inflammation after tooth extraction. These results indicate that diabetic patients have a chronic inflammatory phase, which might affect the ability to heal wounds. Salivary IL-6 and IL-8 can be potentially useful, non-invasive biomarkers for monitoring POI in T2DM patients.
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