GENETIC DETECTION OF ISS, TRAT, AND OMPK36 GENES IN GRAM-NEGATIVE BACTERIA ISOLATED FROM DIFFERENT INFECTIONS IN NAJAF PROVINCE
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Abstract
Background: The increase serum survival (iss) gene is associated with the synthesis of the O-antigen capsule, the transfer protein (traT) gene is linked to the synthesis of the K1 capsule, while the outer membrane ptroteint-36 (ompK36) gene is connected to antibiotic resistance.
Objectives: The current study aimed to detection presence of iss gene, Trat gene, Ompk36 gene in different gram negative isolated.
Materials and Methods: A total of 78 Gram-negative bacterial isolates were obtained from intestinal and extra-intestinal infections. All isolates were cultured on MacConkey agar and identified using the Vitek-2 system. Conventional PCR was employed for the amplification of the iss, traT, and ompK36 genes. Microsoft Excel was used for the statistical analysis of the data.
Results: The iss, traT, and ompK36 genes were detected in the total DNA of 78 isolates from the Enterobacteriaceae family, which included 15 isolates each of E. coli, Klebsiella pneumoniae, Salmonella typhi, and Shigella dysenteriae, 12 isolates of Proteus vulgaris, and 6 isolates of Serratia marcescens. The iss gene was present in 51 out of 78 isolates (65%) at 258 bp, the traT gene was detected in 63 out of 78 isolates (80.7%) at 258 bp, and 45 out of 78 isolates (57.6%) tested positive for the ompK36 gene at 305 bp.
Conclusion: The iss, traT, and ompK36 genes were detected among Enterobacteriaceae species isolated from various clinical infections, with a notably high occurrence in extraintestinal isolates.
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